Purification and biochemical properties of tumor-associated transplantation antigens from methylcholanthrene-induced murine sarcomas.
نویسندگان
چکیده
A tumor-associated transplantation antigen with an apparent molecular weight of 75,000 has been isolated from the cytosol of the BALB/c methylcholanthrene-induced sarcoma, Meth A. The antigen was purified either by preparative electrophoresis in the presence of NaDodSO4 or by immunoaffinity chromatography after hexylamine agarose chromatography, gel filtration, and hydroxylapatite chromatography. The 75-kilodalton (kDal) protein prepared by either of these methods effectively primed BALB/c mice to reject the Meth A tumor; such priming provided no protection against challenge by other independently derived sarcomas of BALB/c origin. A second protein, also 75 kDal, was isolated from the cytosol of the recently derived methylcholanthrene-induced sarcoma CI-4 by essentially the same chromatographic scheme. This protein also was immunogenic in the tumor rejection assay and provided protection only against CI-4 challenge. The antigens purified from the Meth A and CI-4 sarcomas appear to be closely related proteins. Both of them can be purified from the cytosol fraction and can be recognized by a rabbit antiserum prepared against the Meth A 75-kDal protein. The two proteins have approximately the same molecular weight, have similar but not identical amino acid compositions, and differ in their chromatographic behavior on hexylamine agarose and hydroxylapatite as well as in their isoelectric points. These results indicate that the individually specific transplantation antigens found in chemically induced sarcomas may be the products of a single multigene family or somatic derivatives of a single gene.
منابع مشابه
Lack of identity between TSTA and alien antigens of methylcholanthrene-induced murine sarcomas.
متن کامل
CD4+ T-cells from mice immunized to syngeneic sarcomas recognize distinct, non-shared tumor antigens.
We have utilized a newly developed culture system to study the properties of antitumor CD4+ T-cells relevant to the rejection of syngeneic methylcholanthrene sarcomas. Fresh syngeneic dendritic cells prepared from spleen, then pulsed with crude lysates of methylcholanthrene sarcomas, evoke antigen-specific proliferation by CD4+ but not by CD8+ T-cells from tumor-immune mice. Unfractionated sple...
متن کاملCellular immunity to Rous sarcoma in tumor-bearing chickens.
Chickens carrying primary sarcomas induced by SchmidtRuppin Rous virus were studied for possible cellular immunity against the tumor-specific transplantation antigens of those tumors by use of the colony inhibition test. The thymus cells of 7 out of 8 tested chickens were found to reduce signifi cantly the colony formation of Rous sarcoma cells while they had no similar effect on other types of...
متن کاملCell surface antigens of chemically induced fibrosarcomas: detection by a monoclonal antibody of a tumor-restricted Mr 12,000 protein gag antigen encoded by a dual-tropic murine leukemia virus.
Fusion products of spleen cells of W/FuDp rats immunized with a methylcholanthrene-induced BALB/c sarcoma, CA-2, and mouse myeloma cells were screened in an attempt to identify a monoclonal antibody defining the individually distinct tumor-specific transplantation antigen of CA-2. A hybridoma, MP/69/04, was isolated which produces an IgG2a monoclonal antibody that recognized a tumor-restricted ...
متن کاملTumor-specific transplantation antigens in reticulum cell sarcomas and lymphomas induced by the friend virus complex.
The reticulum cell sarcomas induced by Friend leukemia virus (FLV) and the lymphomas induced by the Friend virus-associated lymphatic leukemia virus (LLV) have been investigated for both their antigenic relationships and the presence in them of common tumor-specific transplantation antigens. Since noninfectious reticulum cell sarcomas contain ing the Friend virus genome were available, this was...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 79 24 شماره
صفحات -
تاریخ انتشار 1982